By Webb, Elgood
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This antiserum was tentatively designated as a-FS3. serum samples were collected and stored as mentioned above. 2% agarose (Litex HSA, Denmark) according to Hara et al. (1984), Mancini et al. (1965) and Ouchterlony (1953) respectively. A portion of S. taczanowskii ovary was fixed in Bouin's solution for 2 h and then rinsed in cold PBS (4° C) for 2 days. It was embedded in paraffin (5658° C) and 5/Lm sections were prepared. Sections were stained by the unlabeled antibody immunoperoxidase method as suggested by Nunomura et al.
Station 12: 37-44. (in Japanese). Ouchterlony, O. 1953. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Path. Microbiol. Scand. 32: 231-240. , K. Aida & I. Hanyu. 1985. Endocrine profiles during the short reproductive cycle of an autumn-spawning bitterling, Acheilognathus rhombea. Gen. Compo Endocrinol. 60: 361-371. Shiokawa, T. 1962a. Growth and maturity of the common rock- 48 fish, Sebastiscus marmoratus Cuvier et Valenciennes. Rec. Oceanogr.
Numbers indicate sample sizes. zanowskii are commonly distributed in shallow depths from Iwate Prefecture throughout the coasts of Hokkaido, Japan. Sebastes schlegeli are rather large in size among the Japanese scorpaenids, reaching about 40 cm SL, and can be easily reared in aquaria. weight-I] was calculated. Blood was taken from the caudal vasculature, usually via a nonheparinized syringe with a needle (23 G) or, in some cases, simply by removing the tail. Following centrifugation at 3000 rpm for 15 min, the serum obtained was stored at - 20°C until analysis.
Animal classif by Webb, Elgood